Aggregation of embryonic stem cells induces Nanog ...

These cell-aggregation-based changes in vitro should represent the process of cell-lineage specification in the inner cell mass of blastocysts. Importantly, Nanog repression occurred solely by cell aggregation, independent of the LIF/STAT3 or BMP4 pathways.

Aggregation of Sponge Cells - Journal of Biological …

that cell separation is the consequence of an activation of the cell membrane bound P-glucuronidase, which results in a deglucuronylation of the aggregation recep- tor. Among the different biological models studied to under- stand cell-cell aggregation processes both on the cellular and

Erythrocyte aggregation - Wikipedia

Erythrocyte aggregation is the reversible clumping of red blood cells (RBCs) under low shear forces or at stasis.Erythrocytes aggregate in a special way, forming rouleaux.Rouleaux are stacks of erythrocytes which form because of the unique discoid shape of the cells in vertebrate body. The flat surface of the discoid RBCs give them a large surface area to make contact and stick to each other ...

UNDERSTANDING CONTROL OF XYLELLA FASTIDIOSA CELL ...

aggregation processes as the WT cells. Mutant cells lacking type I pili [fimX (previously designated fimA)] also formed cell aggregates, but the process differed from the WT in that the cells repeatedly aggregated and dispersed, but eventually formed relatively stable, albeit smaller aggregates (Figure 2b).

In-cell Aggregation of a Polyglutamine-containing Chimera ...

Dec 14, 2007· Like the previous picture from in vitro studies, largely in the ataxin-3 system, aggregation in the cell emerges as a multistep process with distinct types of aggregates formed en route to the mature fibrils: 1) a spherical aggregate that can accelerate CRABP aggregation, suggesting a structure with a CRABP core and 2) fibrillar aggregate with ...

87 questions in Cell Aggregation | Science topic

The phenomenon by which dissociated cells intermixed in vitro tend to group themselves with cells of their own type. | Explore the latest articles, projects, and questions and answers in Cell ...

Aggregation of Monoclonal Antibody Products: Formation and ...

Aggregates are typically large, tangled clusters of denatured antibody molecules that are irreversibly formed either during product expression in the cell culture, product purification in downstream processing, or storage as drug substance or drug product (see Figure 1).The process of aggregation is influenced by the biochemical and biophysical properties of the mAb itself, as well as the ...

Guest Editor - Steve Shire Protein Aggregation and ...

Protein aggregation is a common issue encountered during manufacture of biotherapeutics. It is possible to infl uence the amount of aggregate produced during the cell culture and purifi cation process by carefully controlling the envi-ronment (eg, media components) and implementing appro-priate strategies to minimize the extent of aggregation.

Challenges of Protein Aggregation during Purification ...

In addition, protein aggregation can refer to a broad range of molecular behaviors, from dimer formation to visible particulates due to noncovalent interactions mediated by hydrophobicity and covalent interactions through pairing of interchain unpaired thiol groups, according to John M. Liddell, head of process sciences with Fujifilm Diosynth ...

A Computational Model of Chemotaxis-based Cell Aggregation

A Computational Model of Chemotaxis-based Cell Aggregation Manolya Eyiyurekli Advisor: David E. Breen,PhD We present a 2D computational model that successfully captures the cell be-haviors that play important roles in cell aggregation. A virtual cell in our model is designed asanindependent, discrete unit withacollection ofparameters andactions.

Protein aggregation - Wikipedia

Cells have mechanisms that can refold or degrade protein aggregates. However, as cells age, these control mechanisms are weakened and the cell is less able to resolve the aggregates. The hypothesis that protein aggregation is a causative process in aging is testable now since some models of delayed aging are in hand.

Chapter 4 Phenomena of Cellular Recognition in Sponges ...

The existence of these substances offers tentative explanations concerning recognition of "self" vs "not self"; they suggest that morphogenetic cell aggregation and selective cell adhesion are closely related phenomena, at least in those cases when cell contact specificity is displayed early in the process of cell aggregation.

Development of Plant Cell Culture Processes to Produce ...

DEVELOPMENT OF PLANT CELL CULTURE PROCESSES TO PRODUCE NATURAL PRODUCT PHARMACEUTICALS: CHARACTERIZATION, ANALYSIS, AND MODELING OF PLANT CELL AGGREGATION A Dissertation Presented by MARTIN E. KOLEWE Submitted to the Graduate School of the University of Massachusetts Amherst in partial fulfillment of the requirements for the degree of

cell aggregation process - latavernadelre.it

Aggregation of Cells Using Biomaterials and Bioreactors. Cell aggregation is recognised as a crucial process during embryonic develop- ment 3 and cell aggregate formation (embryoid bodies) is an important step in embry- onic stem cell differentiation in vitro .

What does cell aggregation mean? - Definitions.net

Definition of cell aggregation in the Definitions.net dictionary. Meaning of cell aggregation. What does cell aggregation mean? Information and translations of cell aggregation in the most comprehensive dictionary definitions resource on the web.

Planteome: Term Details for "cell aggregation" (GO:0098743)

cell aggregation Ontology biological_process Synonyms None Alternate IDs None Definition The clustering together and adhesion of initially separate cells to form an aggregate. Examples include the clustering of unicellular organisms or blood cells in suspension and the condensation of mesenchymal cells during cartilage formation.

Cell Aggregation - an overview | ScienceDirect Topics

While this method is suitable for aggregation of many cells, serum or serum proteins must be added to promote cell aggregation in many cases, thus making it difficult to characterize the aggregation process and to control the size and composition of the aggregate. Specialized techniques can be used to produce aggregates in certain cases ...

Cell Aggregation Assays – Precise Viability and Cell Counting

The NucleoCounter ® NC-200™ counts nucleated cells, by fluorescent staining of the DNA ensuring only viable cells are counted. With a wide range of specialized assays, the NC-200™ is the perfect cell counter. Sphere cultures or breast cancer cell lines poses great challenges to cell …

A Computational Model of Chemotaxis-based Cell …

A Computational Model of Chemotaxis-based Cell Aggregation Manolya Eyiyurekli Advisor: David E. Breen,PhD We present a 2D computational model that successfully captures the cell be-haviors that play important roles in cell aggregation. A virtual cell in our model is designed asanindependent, discrete unit withacollection ofparameters andactions.

THE EFFECTS OF SURFACTANTS ON CELL AGGREGATION

Surfactants and cell aggregation 367 hering HeLa cells. To test this hypothesis the effect of shear rate on the reaggregation process was investigated, by means of measurements of aggregation in a Couette viscometer in which linear shear at known and controlled rates could be applied. Dissociated HeLa cells were reaggregated in Eagle's MEM.

Characterization of mAb aggregates in a mammalian cell ...

Dec 04, 2013· Protein aggregation is a major concern during monoclonal antibody (mAb) production [1, 2].The presence of aggregates can reduce the therapeutic efficacy of mAbs and trigger immunogenic responses upon administration [].Higher molecular weight (HMW) aggregates can be removed during downstream processing (DSP), but prevention of aggregate formation upstream could increase process …

The growing complexity of platelet aggregation | Blood Journal

Platelet aggregation, the process by which platelets adhere to each other at sites of vascular injury, has long been recognized as critical for hemostatic plug formation and thrombosis. Until relatively recently, platelet aggregation was considered a straightforward process involving the noncovalent bridging of integrin αIIbβ3 receptors on the platelet surface by the dimeric adhesive protein ...

The effect of red blood cell aggregation and how it can be ...

The effect of red blood cell aggregation and how it can be reversed T Lew Lim, MedicLights Research Inc., Toronto, Canada. May 2010 Introduction The blood profile reveals a person's state of health. If we look at a blood sample of an unhealthy subject, we will see that the red blood cells (RBCs) are sticking together (aggregating) and

A Computational System for Investigating Chemotaxis …

A Computational System for Investigating Chemotaxis-Based Cell Aggregation Manolya Eyiyurekli 1Peter I. Lelkes2 David E. Breen 1Department of Computer Science, College of Engineering 2School of Biomedical Engineering, Science and Health Systems Drexel …

Identification of process parameters influencing product ...

Dec 14, 2015· Finally, we have identified important process parameters to influencing protein aggregation in mammalian cell culture. Hence, our work demonstrates that the formation of mAb aggregates can be assessed directly in mammalian cell culture and product quality can be controlled by the selection of certain cell culture process parameters.

An Efficient and Flexible Cell Aggregation Method for 3D ...

Cells are prepared as described in Figure 1, but are resuspended in spheroid formation medium containing a minimum of 30 mg/mL MC which forms a thick layer to hold individual cells in suspension and prevent cell aggregation (i-ii). The cell suspension is seeded into U-bottom cell-repellent plates and …

Mathematical Model for the Process of Aggregation in ...

Mathematical Model for the Process of Aggregation in Immune Agglutination ... collision of red cells resulting in the formation of antibody cross-linkages between cells and visible aggregation 1,2.

Agglutination | definition of agglutination by Medical ...

agglutination [ah-gloo″tĭ-na´shun] 1. the action of an agglutinant substance. 2. the clumping together in suspension of antigen-bearing cells, microorganisms, or particles in the presence of specific antibodies (agglutinins). Agglutination reactions. From Applegate, 2000. 3. the process of union of the surfaces of a wound. adj., adj agglutina´tive ...